| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Oakville High School | A | Mueller | SQUIRES | WESCOAT | JONAS | 55.83 | Verified |
| 2 | Mehlville High School | A | Abdijanovic | Roberts | O'Shea | Palmer | 53.94 | Verified |
| 3 | Ritenour High School | A | Brantley | McGhee | Jones | French | 51.27 | Verified |
| 4 | Eureka High School | A | 50.91 | Verified | ||||
| 5 | Lafayette (Wildwood) High School | A | 51.24 | Verified | ||||
| 6 | Parkway South High School | A | Bacon | Stroot | Wilson | Wright | 53.01 | Verified |
| 7 | Fox High School | A | Trompeter | Rogers | Carter | West | 54.29 | Verified |