| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mehlville High School | A | Traore | Raymonde Jean | Sutherland | Cohen-Edwards | 1:03.10 | Unverified: |
| 2 | Fox High School | A | Wiley | Butler | Norwood | Masters | 54.29 | Verified |
| 3 | Lafayette (Wildwood) High School | A | 53.83 | Unverified: | ||||
| 4 | Ritenour High School | A | Royal | Phillips | Davis | Shaw | 51.27 | Verified |
| 5 | Parkway South High School | A | Cashion | Gavornik | McHale | Scott | 53.01 | Verified |
| 6 | Eureka High School | A | KIEFER | Bailey | Thiele | FRAZIER | 53.96 | Unverified: Kimball/Wintermeyer Girls Invite |
| 7 | Oakville High School | A | Lyons | Pleimann | Reed | 55.83 | Verified |