| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BARNARD, Elissa | SR | Lafayette (Wildwood) High School | 5:02.18 | Verified |
| 2 | BARNARD, Natalie | JR | Lafayette (Wildwood) High School | 5:09.49 | Verified |
| 3 | MARCUM, Isabella | SR | Fox High School | 5:09.64 | Verified |
| 4 | WHITAKER, Kennedy | SR | Parkway West High School | 5:10.04 | Verified |
| 5 | SINGLETON, Magdalena | SR | Parkway South High School | 5:23.31 | Verified |
| 6 | ETCHASON, Alexandra | SR | Parkway West High School | 5:26.29 | Verified |
| 7 | PEARCE, OLIVIA | SO | Eureka High School | 5:26.56 | Verified |
| 8 | MILLER, Allison | SO | Mehlville High School | 5:38.64 | Verified |
| 9 | LENA, Natalie | FR | Parkway South High School | 5:41.23 | Verified |
| 10 | SMITH, Abigail | FR | Eureka High School | 5:43.90 | Verified |
| 11 | KECK, Emily | SR | Oakville High School | 5:59.28 | Verified |
| 12 | BARRETT, Kayla | SO | Mehlville High School | 6:02.95 | Verified |
| 13 | WEISSLER, Elizabeth | JR | Ritenour High School | 6:19.35 | Verified |
| 14 | GUTIERREZ GAONA, Adriana | SR | Ritenour High School | 6:59.26 | Verified |
| 15 | SCOTT, Lily | SR | Fox High School | NT | Unverified: |