| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Brandt | Gerber | Keith | Sakhelashvili | 1:39.06 | Verified |
| 2 | Marquette High School | A | BONNETT | Jones | WILLIAMS | CODY-JACKSON | 1:33.74 | Verified |
| 3 | Seckman High School | A | Gossett | Horton | Harp | Rudanovich | 1:33.19 | Verified |
| 4 | Pattonville High School | A | Eleby | Clark | Adams | Jenkins Jr | 1:28.75 | Unverified: |
| 5 | Hazelwood Central High School | A | Johnson | Martin | Williams | Atkins | 1:30.42 | Verified |
| 6 | Kirkwood High School | A | Welch | Marion | Webb | Edwards | 1:33.50 | Verified |
| 7 | Lindbergh High School | A | Fauth | Earley | Goss | Wightman | 1:35.22 | Verified |