| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Marquette High School | A | Derstine | Small | Bergoudian | JONES | 1:40.20 | Unverified: |
| 2 | Kirkwood High School | A | McKee | Cauble | Walter | Boswinkel | 1:37.50 | Unverified: Dale Collier/Kirkwood Invitation |
| 3 | Hazelwood West High School | A | 1:33.56 | Verified | ||||
| 4 | Hazelwood Central High School | A | George | Gunn | Thornton | Burns | 1:30.42 | Verified |
| 5 | Pattonville High School | A | Redden | Rice | Shannon | Blair | 1:31.75 | Verified |
| 6 | Lindbergh High School | A | Runge | Sengupta | Brown | Engbert | 1:35.22 | Verified |
| 7 | Northwest (Cedar Hill) High Scho | A | Grellner | Huttenlocker | Campbell | Frazier | 1:39.06 | Verified |
| 8 | Seckman High School | A | Reelitz | Dawson | Dibbert | Bruenger | 1:45.27 | Unverified: Simpson Relays |