| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Poskin | Gant | Smith | Sadler | 3:49.26 | Verified |
| 2 | Marquette High School | A | BONNETT | ANDERSON | WILLIAMS | LEOPANDO | 3:34.65 | Verified |
| 3 | Kirkwood High School | A | Stevener | Welch | Birmingham | Snyder | 3:30.18 | Verified |
| 4 | Hazelwood Central High School | A | Johnson | Logan | Hunter | Anthony | 3:25.54 | Verified |
| 5 | Pattonville High School | A | Hayes | Jenkins Jr | Vogler | Seals Jr | 3:28.42 | Unverified: |
| 6 | Seckman High School | A | Gossett | Matus | Tilley | Rudanovich | 3:32.17 | Verified |
| 7 | Lindbergh High School | A | Fauth | Deterding | Goss | Wible | 3:44.02 | Verified |