| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Grellner | Huttenlocker | Penberthy | Frazier | 3:49.26 | Verified |
| 2 | Kirkwood High School | A | McKee | Richman | Araneta | Earl | 3:45.00 | Unverified: |
| 3 | Hazelwood West High School | A | 3:37.63 | Verified | ||||
| 4 | Pattonville High School | A | Cobbs | Chambers | Lewis | Kadima | 3:30.42 | Verified |
| 5 | Hazelwood Central High School | A | Johnson | Bright | Williams | Burns | 3:31.54 | Unverified: Marion Freeman Friday Night Ligh |
| 6 | Lindbergh High School | A | Chavez | Hilton | Engbert | Jeanmougin | 3:44.02 | Verified |
| 7 | Marquette High School | A | Derstine | VALENTINE WALLS | Bergoudian | Small | 3:47.20 | Unverified: |
| 8 | Seckman High School | A | Schutte | Smith | Head | Terry | 3:52.00 | Unverified: |