| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FREUND, Milo | JR | Lindbergh High School | NT | Unverified: |
| 2 | GUNN, Antoine | FR | Hazelwood Central High School | NT | Unverified: |
| 3 | HILTON, Wyatt | SO | Lindbergh High School | NT | Unverified: |
| 4 | HOPGOOD, Martinelle | SO | Pattonville High School | 28.87 | Verified |
| 5 | WEBB, Kamal | JR | Kirkwood High School | 29.56 | Verified |
| 6 | ARNOLD, Chase | SO | Lindbergh High School | NT | Unverified: |
| 7 | GARNER, Daveon | JR | Pattonville High School | NT | Unverified: |
| 8 | REDDEN, TAHJ | SR | Marquette High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ESTRADA, Samuel | SO | Lindbergh High School | 27.44 | Verified |
| 2 | VANA, Nathan | FR | Marquette High School | 26.84 | Verified |
| 3 | RAMPEY, NATHAN | JR | Marquette High School | 26.67 | Verified |
| 4 | STEUBY, Thomas | SO | Kirkwood High School | 25.51 | Verified |
| 5 | NAGO, Casey | FR | Seckman High School | 25.83 | Verified |
| 6 | SMITH, Bailey | FR | Northwest (Cedar Hill) High Scho | 26.75 | Verified |
| 7 | DIBBERT, Seth | JR | Seckman High School | 27.27 | Verified |
| 8 | REELITZ, Christopher | SO | Seckman High School | 28.02 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | ROQUE, MATTHEW | SO | Marquette High School | 25.34 | Verified |
| 2 | BLAIR, Jayden | SO | Pattonville High School | 25.14 | Verified |
| 3 | CAUBLE, Benjamin | SR | Kirkwood High School | 24.78 | Verified |
| 4 | FRAZIER, Omarion | FR | Northwest (Cedar Hill) High Scho | 24.65 | Verified |
| 5 | MRAZ, Mason | FR | Seckman High School | 24.71 | Verified |
| 6 | WEBSTER, Amani | SR | Hazelwood West High School | 24.88 | Verified |
| 7 | SANKPAL, Aryan | JR | Pattonville High School | 25.26 | Verified |
| 8 | GRELLNER, Andrew | SO | Northwest (Cedar Hill) High Scho | 25.36 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUTTENLOCKER, Dalton | SO | Northwest (Cedar Hill) High Scho | 24.37 | Verified |
| 2 | ANTHONY, Dontea | SO | Hazelwood Central High School | 24.30 | Verified |
| 3 | JOHNSON, Daron | SR | Hazelwood West High School | 24.11 | Verified |
| 4 | ABDUL-MUMIN, Amel | SR | Hazelwood West High School | 23.58 | Verified |
| 5 | BOSWINKEL, Laurens | JR | Kirkwood High School | 24.03 | Verified |
| 6 | JONES, Camarion | JR | Hazelwood Central High School | 24.27 | Verified |
| 7 | JOHNSON, Ryan | SR | Hazelwood West High School | 24.37 | Verified |
| 8 | BRIGHT, Alan | SO | Hazelwood Central High School | 24.50 | Verified |