| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SPERATO, Graham | FR | Kirkwood High School | 2:25.34 | Verified |
| 2 | UMFLEET, Owen | SR | Seckman High School | 2:27.00 | Unverified: |
| 3 | JONES, Nathaniel | SR | Hazelwood West High School | 2:29.35 | Verified |
| 4 | SCHELLER, William | SO | Northwest (Cedar Hill) High Scho | 2:29.57 | Verified |
| 5 | FEAMAN, Gabriel | SO | Hazelwood Central High School | 2:30.37 | Verified |
| 6 | RILEY, KENNEDY | SO | Marquette High School | 2:32.38 | Verified |
| 7 | LAIRD, John | FR | Kirkwood High School | 2:32.73 | Verified |
| 8 | MUELLER, Liam | SO | Lindbergh High School | 2:38.00 | Verified |
| 9 | MASSAY, Aaron | FR | Pattonville High School | 2:42.32 | Verified |
| 10 | HARRIS, Brendan | FR | Pattonville High School | 2:50.15 | Verified |
| 11 | JUDGE, Matthew | FR | Pattonville High School | 2:54.29 | Verified |
| 12 | JENNING, Elijah | FR | Seckman High School | 2:54.82 | Verified |
| 13 | DAVIS, Sir'Rickey | FR | Hazelwood West High School | NT | Unverified: |
| 14 | WEBSTER, Anthony | SO | Hazelwood Central High School | NT | Unverified: |
| 15 | WOOLFOLK, Cashius | JR | Hazelwood Central High School | NT | Unverified: |
| 16 | MCCARRON, Jobe | FR | Pattonville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | FERKEL, Peyton | SO | Kirkwood High School | 2:07.06 | Verified |
| 2 | SCHUTTE, Dominic | JR | Seckman High School | 2:08.00 | Unverified: |
| 3 | RICHMAN, George | SO | Kirkwood High School | 2:08.90 | Verified |
| 4 | PORTER, Evan | SO | Northwest (Cedar Hill) High Scho | 2:11.21 | Verified |
| 5 | BARTON, Jacob | FR | Marquette High School | 2:13.61 | Verified |
| 6 | SMITH, Sebastian | SO | Seckman High School | 2:13.93 | Verified |
| 7 | MWANGI, Frank | FR | Hazelwood West High School | 2:16.66 | Verified |
| 8 | TOLES, Larry | SR | Hazelwood Central High School | 2:21.27 | Verified |
| 9 | JEANMOUGIN, Nathaniel | SO | Lindbergh High School | 2:21.61 | Verified |
| 10 | JUDGE, Jackson | FR | Northwest (Cedar Hill) High Scho | 2:21.66 | Verified |
| 11 | BURKE, ROYCE | SO | Marquette High School | 2:21.82 | Verified |
| 12 | MEIER, Luke | SO | Northwest (Cedar Hill) High Scho | 2:22.67 | Verified |
| 13 | HAMPTON, Colton | FR | Lindbergh High School | 2:23.39 | Verified |
| 14 | MENEFEE, DANIEL | SO | Marquette High School | 2:24.00 | Verified |
| 15 | JEANMOUGIN, Evan | FR | Lindbergh High School | 2:24.40 | Verified |
| 16 | BURROW, Ronrecus | SR | Hazelwood West High School | 2:24.53 | Verified |