| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILSON, Tyler | JR | Northwest (Cedar Hill) High Scho | NT | Unverified: |
| 2 | PHILLIPS, Dominick | JR | Hazelwood West High School | 1:00.71 | Verified |
| 3 | WARD, Michael | SR | Seckman High School | 59.85 | Verified |
| 4 | GOSS, Zachary | JR | Lindbergh High School | 54.41 | Verified |
| 5 | LIEBETREU, Nathan | SR | Lindbergh High School | 57.14 | Verified |
| 6 | SMITH, Lucas | JR | Northwest (Cedar Hill) High Scho | 1:00.06 | Verified |
| 7 | HOLMES, Devin | SO | Hazelwood West High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | VOGLER, Donovan | SR | Pattonville High School | 53.87 | Verified |
| 2 | SLAUGHTER, Brandon | JR | Pattonville High School | 52.35 | Unverified: Dale Collier Invitational |
| 3 | JOHNSON, Larry | SR | Hazelwood Central High School | 50.40 | Verified |
| 4 | CODY-JACKSON, CALEB | SR | Marquette High School | 48.87 | Verified |
| 5 | ANTHONY, Deontae | SO | Hazelwood Central High School | 50.27 | Verified |
| 6 | RUDANOVICH, Kaleb | SR | Seckman High School | 51.71 | Verified |
| 7 | WEBB, Khalil | SR | Kirkwood High School | 53.72 | Verified |
| 8 | WELCH, Jaylon | SR | Kirkwood High School | 53.89 | Verified |