| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Brandt | Keith | Sakhelashvili | Vitale | 47.68 | Verified |
| 2 | Marquette High School | A | WILLIAMS | BONNETT | Jones | CODY-JACKSON | 45.17 | Verified |
| 3 | Seckman High School | A | Harp | Tilley | Gossett | Horton | 44.60 | Verified |
| 4 | Pattonville High School | A | Eleby | Clark | Kedl Thomas | Adams | 43.18 | Unverified: |
| 5 | Hazelwood Central High School | A | Johnson | Atkins | Martin | Anthony | 43.28 | Verified |
| 6 | Kirkwood High School | A | Sloan | Edwards | Marion | Richardson | 44.67 | Verified |
| 7 | Lindbergh High School | A | Earley | Watts | Eaton | Lane | 46.00 | Verified |