| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Northwest (Cedar Hill) High Scho | A | Grellner | Huttenlocker | Penberthy | Frazier | 47.68 | Verified |
| 2 | Lindbergh High School | A | Estrada | Runge | Russo | Frantti | 46.00 | Verified |
| 3 | Pattonville High School | A | Porter | Redden | Rice | Blair | 44.48 | Verified |
| 4 | Hazelwood Central High School | A | Anthony | Burns | Bright | Thornton | 43.28 | Verified |
| 5 | Hazelwood West High School | A | 44.37 | Verified | ||||
| 6 | Kirkwood High School | A | Griffin | Boswinkel | Earl | McKee | 45.80 | Unverified: |
| 7 | Seckman High School | A | Mraz | Stinchcomb | Johnston | Nago | 47.33 | Unverified: McCullough-Douglas Invitational |
| 8 | Marquette High School | A | COLE | Clark | REDDEN | ROQUE | 48.10 | Unverified: |