| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOUSE, James | JR | Seckman High School | 4:26.58 | Verified |
| 2 | STEVENER, Graham | SO | Kirkwood High School | 4:32.18 | Verified |
| 3 | STEVENSON, Luke | JR | Pattonville High School | 4:32.86 | Verified |
| 4 | WIBLE, Alvin | JR | Lindbergh High School | 4:37.17 | Verified |
| 5 | HICKMAN, Andrew | SR | Kirkwood High School | 4:43.11 | Verified |
| 6 | KERSHAW, Michael | SO | Northwest (Cedar Hill) High Scho | 4:47.15 | Verified |
| 7 | HUMMEL, Willem | JR | Marquette High School | 4:49.31 | Verified |
| 8 | EVANS, TJ | JR | Seckman High School | 4:50.64 | Verified |
| 9 | OTTO, Brennan | SO | Pattonville High School | 4:52.55 | Verified |
| 10 | WINKA, John | SR | Marquette High School | 4:58.65 | Verified |
| 12 | SWEENEY, Colin | JR | Lindbergh High School | 5:05.74 | Verified |
| 13 | HERRIN, Carter | JR | Northwest (Cedar Hill) High Scho | 5:07.93 | Verified |