| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Belle High School | A | Bailey | Seay | Gromowski | Gromowski | 1:45.96 | Verified |
| 4 | Cuba High School | A | DeClue | Halbert | Laney | Bonney | 1:41.46 | Verified |
| 5 | St. James High School | A | Bell | Crocker | Hancock | Bierman | 1:43.12 | Verified |