| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Greenville High School | A | DeSpain | Elston | Helm | Thiele | 2:08.26 | Verified |
| 4 | St. James High School | A | Freeman | Deardeuff | Foust | Campbell | 1:57.91 | Verified |
| 5 | Cuba High School | B | Lunsford | Martellaro | Moreland | Black | 2:04.58 | Verified |
| 6 | Cuba High School | A | DeLuna | Ringeisen | Osentoski | West | 2:17.57 | Unverified: Montgomery County Invitational |