| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Greenville High School | A | Hopkins | Huff | Irions | Marler | 4:02.39 | Verified |
| 4 | Cuba High School | A | Dake | Halbert | Volner | Hinojosa | 3:48.44 | Verified |
| 5 | St. James High School | A | Altis | Crocker | Siders | 3:55.04 | Verified | |
| 6 | Newburg High School | A | Birdsong | Morrow | Turner | Decker | 4:19.65 | Verified |