| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Greenville High School | A | DeSpain | Elston | Helm | Thiele | 5:39.50 | Verified |
| 3 | Cuba High School | A | Hardcastle | Ijames | Black | Weber | 4:55.21 | Verified |
| 4 | Steelville High School | A | Turnbough | Collinson | Pryor | Brooks | 4:38.13 | Verified |
| 5 | Newburg High School | A | Brinkley | Caquelin | Fite | Twyman | 4:54.65 | Verified |
| 6 | St. James High School | A | Deardeuff | Freeman | Snyder | Goodin | 5:06.90 | Verified |