| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | CONAWAY, Jorja | JR | Viburnum High School | NT | Unverified: |
| 4 | WEST, Averie | SR | Cuba High School | 35.67 | Verified |
| 5 | ELSTON, Chloe | FR | Greenville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BARCOMB, Emilea | FR | St. James High School | 34.81 | Verified |
| 3 | HOLT, Marissa | SO | St. James High School | 33.09 | Verified |
| 4 | WISHON, Kylie | FR | Steelville High School | 32.62 | Verified |
| 5 | THIELE, Chaney | JR | Greenville High School | 32.94 | Verified |
| 6 | WIDMER-ROBERTS, Alexis | JR | Greenville High School | 33.53 | Verified |
| 7 | LOGAN, Caitlin | SO | Newburg High School | 34.85 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PYATT, Lanee | JR | Viburnum High School | 31.61 | Verified |
| 2 | PRATT, Ciarra | JR | Newburg High School | 31.02 | Verified |
| 3 | FREEMAN, Alexus | JR | St. James High School | 30.37 | Verified |
| 4 | NABER, Hali | SR | Belle High School | 27.81 | Verified |
| 5 | CAMPBELL, Avery | FR | St. James High School | 28.54 | Verified |
| 6 | BLACK, Rylee | JR | Cuba High School | 30.61 | Verified |
| 7 | DUNHAM, Hannah | JR | Cuba High School | 31.48 | Verified |
| 8 | LUNSFORD, Ciara | JR | Cuba High School | 31.98 | Verified |