| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BAILEY, Curtis | JR | Steelville High School | 2:28.64 | Verified |
| 2 | MORELAND, Austin | JR | Steelville High School | 2:29.36 | Verified |
| 3 | FINNEGAN, Barrett | SO | Belle High School | 2:31.34 | Verified |
| 4 | PERKINS, Johnny | FR | St. James High School | 2:37.71 | Verified |
| 5 | HOPKINS, Tracen | JR | Greenville High School | 2:38.20 | Verified |
| 6 | HARRIS, Tyson | JR | Steelville High School | 2:41.14 | Verified |
| 7 | SMITH, Jeremy | FR | Cuba High School | 2:41.31 | Verified |
| 8 | HOEHNE, Adrien | JR | Belle High School | 3:02.73 | Verified |