| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CAQUELIN, Akaela | SO | Newburg High School | 2:33.92 | Verified |
| 2 | TURNBOUGH, Audrey | JR | Steelville High School | 2:40.19 | Verified |
| 3 | HARDCASTLE, Patricia | FR | Cuba High School | 2:47.63 | Verified |
| 4 | IJAMES, Izzabelle | JR | Cuba High School | 2:47.79 | Verified |
| 5 | COLLINSON, Aleah | JR | Steelville High School | 2:50.34 | Verified |
| 6 | FITE, Brooklyn | FR | Newburg High School | 2:51.03 | Verified |
| 7 | WOOLS, Kailey | SO | Cuba High School | 3:05.59 | Verified |
| 8 | WEST, Ariel | FR | Greenville High School | 3:07.75 | Verified |