| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | HENNE, Hailey | SO | Steelville High School | NT | Unverified: |
| 4 | DEARDEUFF, Erika | SR | St. James High School | 1:01.09 | Verified |
| 5 | MARTELLARO, Rachel | SO | Cuba High School | 1:02.65 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BROOKS, Peyton | FR | Steelville High School | 1:00.93 | Verified |
| 2 | SNYDER, Ava | FR | St. James High School | 1:00.39 | Verified |
| 3 | WEST, Ashlyn | JR | Greenville High School | 54.36 | Verified |
| 4 | TWYMAN, Corra | SR | Newburg High School | 50.31 | Verified |
| 5 | WIGGER, Natalie | SO | Viburnum High School | 53.42 | Verified |
| 6 | WEST, Ariel | FR | Greenville High School | 59.91 | Verified |
| 7 | EVANS, Norah | JR | Steelville High School | 1:00.65 | Verified |