| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOEHNE, Adrien | JR | Belle High School | 1:16.63 | Verified |
| 2 | TAUSCHER, Marshall | FR | Viburnum High School | 1:10.67 | Verified |
| 3 | HERR, Kaleb | FR | Cuba High School | 1:07.47 | Verified |
| 4 | SMITH, Jeremy | FR | Cuba High School | 1:04.78 | Verified |
| 5 | SETZER, Boston | SO | Steelville High School | 1:06.15 | Verified |
| 6 | SEAY, Camrin | SO | Belle High School | 1:09.78 | Verified |
| 7 | KINSEY, Kaegan | FR | Belle High School | 1:15.99 | Verified |
| 8 | HARDIN, Wyatt | SO | Steelville High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTIN, Alex | FR | Steelville High School | 1:00.54 | Verified |
| 2 | SIDERS, Brandon | FR | St. James High School | 59.94 | Verified |
| 3 | MORROW, Evin | FR | Newburg High School | 59.07 | Verified |
| 4 | MIDYETT, Jacob | JR | Steelville High School | 53.85 | Unverified: |
| 5 | HUFF, Jeb | SR | Greenville High School | 55.74 | Verified |
| 6 | HARDY, Collin | JR | Viburnum High School | 59.49 | Verified |
| 7 | HOPKINS, Tracen | JR | Greenville High School | 1:00.34 | Verified |
| 8 | IRIONS, Carter | SR | Greenville High School | 1:01.95 | Verified |