| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 3 | FELAN, Addalye | FR | St. James High School | 1:28.51 | Verified |
| 4 | LOGAN, Caitlin | SO | Newburg High School | 1:15.75 | Verified |
| 5 | PRATT, Ciarra | JR | Newburg High School | 1:18.95 | Verified |
| 6 | ROSA, Mary | JR | Newburg High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WOOLS, Kailey | SO | Cuba High School | 1:14.28 | Verified |
| 2 | CONAWAY, Jorja | JR | Viburnum High School | 1:10.88 | Verified |
| 3 | TURNBOUGH, Audrey | JR | Steelville High School | 1:08.67 | Unverified: |
| 4 | DESPAIN, Briannna | JR | Greenville High School | 1:00.51 | Verified |
| 5 | FOUST, Jaci | FR | St. James High School | 1:07.34 | Verified |
| 6 | HARDCASTLE, Patricia | FR | Cuba High School | 1:10.19 | Verified |
| 7 | IJAMES, Izzabelle | JR | Cuba High School | 1:12.15 | Verified |
| 8 | COLLINSON, Aleah | JR | Steelville High School | 1:14.97 | Verified |