| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Belle High School | B | Bailey | Seay | Butler | Gromowski | 51.70 | Verified |
| 3 | St. James High School | B | Strong-Madie | Pearson | Salmons | Bierman | NT | Unverified: |
| 4 | St. James High School | A | Spurgeon | Altis | Noakes | Snyder | 45.35 | Verified |
| 5 | Cuba High School | A | Dake | Volner | Laney | Hinojosa | 46.82 | Verified |
| 6 | Belle High School | A | Weigel | Gromowski | Valley | Smith | 51.70 | Verified |