| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HUFF, Jeb | SR | Greenville High School | 4:59.62 | Verified |
| 2 | SHIELDS, Luke | JR | St. James High School | 5:12.96 | Verified |
| 3 | SMITH, Baylar | JR | Belle High School | 5:13.75 | Verified |
| 4 | BAILEY, Curtis | JR | Steelville High School | 5:21.26 | Verified |
| 5 | FINNEGAN, Barrett | SO | Belle High School | 5:37.90 | Verified |
| 6 | BROOKS, Gabriel | JR | Viburnum High School | 5:38.08 | Verified |
| 7 | MORELAND, Austin | JR | Steelville High School | 5:42.01 | Verified |
| 8 | HILL, Jackson | JR | Greenville High School | 5:44.44 | Verified |
| 9 | PERKINS, Johnny | FR | St. James High School | 5:49.78 | Verified |
| 10 | HARRIS, Tyson | JR | Steelville High School | 6:21.60 | Verified |
| 11 | DECKER, Alex | JR | Newburg High School | 6:27.32 | Verified |
| 12 | WEIGEL, Connor | FR | Belle High School | NT | Unverified: |