| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | HOLTMEYER, Zachary | 8 | Owensville Middle School | 5:23.91 | Verified |
| 2 | BAKER, Tandon | 8 | St. James Middle School | 6:02.90 | Verified |
| 3 | PERKINS, Braden | 8 | Owensville Middle School | 6:07.58 | Verified |
| 4 | MILES, Granite | 8 | Owensville Middle School | 6:09.54 | Verified |
| 5 | HAZELWOOD, Coltin | 6 | Bland Middle School | 6:11.49 | Verified |
| 6 | ROBERTSON, Brice | 8 | Bland Middle School | 6:11.74 | Verified |
| 7 | WEIBLE, Caden | 8 | Newburg Middle School | 6:22.00 | Unverified: |
| 8 | WALLIS, Garrett | 7 | Bourbon Middle School | 6:33.82 | Verified |
| 9 | DIXON, Maddox | 7 | Viburnum Junior High School | 6:36.34 | Verified |
| 10 | RUTLEDGE, Owen | 8 | Steelville Middle School | 6:39.13 | Verified |
| 11 | SIEFERT, Karsin | 8 | Bland Middle School | 6:43.27 | Verified |
| 12 | KING, Asher | 6 | Bourbon Middle School | 6:47.97 | Verified |
| 13 | TIMM, Dalton | 7 | Vienna Middle School | 7:00.34 | Verified |
| 14 | CRANDLE, Blakely | 6 | Vienna Middle School | 8:00.00 | Unverified: |
| 15 | MORELAND, Ashton | 7 | St. James Middle School | NT | Unverified: |
| 16 | BROWN, Caleb | 7 | Cuba Middle School | NT | Unverified: |
| 17 | DELUNA, Christian | 7 | Cuba Middle School | NT | Unverified: |
| 18 | DUNLAP, Ross | 8 | Viburnum Junior High School | NT | Unverified: |
| 19 | MATHENA, Judah | 8 | Cuba Middle School | NT | Unverified: |
| 20 | HUTCHCRAFT, Ryder | 7 | St. James Middle School | NT | Unverified: |
| 21 | MOUSADAKOS, Vasilis | 7 | Rolla MS | NT | Unverified: |
| 22 | FISCHER, Sebastian | 7 | Rolla MS | NT | Unverified: |
| 23 | CAMERON, Cody | 7 | Rolla MS | NT | Unverified: |