| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Vienna High School | B | Hamill | Kilmer | Wulff | Zimmer | 1:49.22 | Verified |
| 3 | Vienna High School | A | Baz | Hayes | Kleffner | Schwartze | 1:44.50 | Unverified: Linn High School Invitational |
| 4 | Rolla High School | A | Thomas | Stinson | Kearse | Stallworth | 1:37.02 | Verified |
| 5 | Rolla High School | B | Briggs | Harris | Haas | Plank | DQ | Unverified: Jefferson City & Helias & Rolla |
| 6 | St. James High School | A | Bullock | Bell | Earls | Hancock | 1:46.65 | Verified |
| 7 | Salem High School | A | Campbell | Mendenhall | Koontz | Vernellus | NT | Unverified: |