| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Viburnum High School | A | Pyatt | Halbert | Conaway | Wigger | 2:07.96 | Verified |
| 3 | Iberia High School | A | Luttrell | Pemberton | Russell | Long | 2:00.88 | Verified |
| 4 | Rolla High School | A | Toney | Inskip | Alamaldein | Kriete | 1:57.35 | Verified |
| 5 | Vienna High School | A | Weeks | Novak | Schell | Reeves | 2:00.28 | Verified |
| 6 | St. James High School | A | Snyder | Deardeuff | Foust | Campbell | 2:02.38 | Verified |