| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Iberia High School | A | Spencer | VanVleck | Mace | Parker | 4:11.62 | Verified |
| 4 | Vienna High School | A | Kilmer | Wilkinson | Baz | Douglas | 4:04.50 | Unverified: Eugene HS Invitational |
| 5 | Vienna High School | B | Hayes | Kleffner | Schwartze | Wulff | 4:08.14 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St. Clair High School | A | Bekemeier | Sanders | Blake | Arndt | 4:03.94 | Verified |
| 2 | St. James High School | A | Siders | Bullock | Crocker | Shields | 4:02.00 | Unverified: |
| 3 | Salem High School | B | Gately | May | Campbell | Mendenhall | 3:53.89 | Verified |
| 4 | Rolla High School | A | Stinson | Lortz | Hamer | Briggs | 3:45.97 | Verified |
| 5 | Rolla High School | B | Simmons | Lindsey | Barr | Shenefield | 3:49.00 | Unverified: Jefferson City & Helias & Rolla |
| 6 | Salem High School | A | Radford | Lewis | Atkinson | Green | 3:53.89 | Verified |
| 7 | St. Clair High School | B | Hawkins | Folks | Rulo | Sohn | 4:03.94 | Verified |