| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Rolla High School | A | Lortz | Aguilarleon | Shenefield | Pratz | 9:04.87 | Verified |
| 2 | Rolla High School | B | Huckabee | Lindsey | Williams | Alderfer | 9:10.87 | Unverified: Jefferson City & Helias & Rolla |
| 3 | St. Clair High School | A | Kern | James | Yarberry | Perkins | 9:17.64 | Verified |
| 4 | Vienna High School | A | Auten | Wilkinson | Ewers | Kilmer | 9:28.40 | Verified |
| 5 | Salem High School | A | Atkinson | Diehl | Radford | Green | NT | Unverified: |
| 6 | Salem High School | B | Brown | Parks | Sherman | Thies | NT | Unverified: |