| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Vienna High School | B | Crum | Camden | Rollins | Carnes | 58.40 | Unverified: |
| 2 | St. James High School | B | Deluca | Salmons | Bierman | Strong-Madie | NT | Verified |
| 3 | Rolla High School | B | Plank | Price | Harris | Stinson | 47.00 | Unverified: Jefferson City & Helias & Rolla |
| 4 | Salem High School | A | Campbell | Mendenhall | Owen | Vernellus | 46.02 | Verified |
| 5 | Rolla High School | A | Haas | Mertens | Kearse | Stallworth | 46.06 | Verified |
| 6 | Vienna High School | A | Baz | Kleffner | Schwartze | Hayes | 47.80 | Unverified: Eugene HS Invitational |
| 7 | St. James High School | A | Spurgeon | Earls | Noakes | Bell | 48.17 | Verified |