| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | MARTENSEN, Cameron | FR | Rolla High School | 4:28.78 | Verified |
| 2 | WILKINSON, Duncan | JR | Vienna High School | 4:57.99 | Verified |
| 3 | PERKINS, Thomas | JR | St. Clair High School | 5:01.89 | Verified |
| 4 | AUTEN, Cooper | SO | Vienna High School | 5:05.25 | Verified |
| 5 | AGUILARLEON, Nathaniel | SO | Rolla High School | 5:09.10 | Verified |
| 6 | SHIELDS, Luke | JR | St. James High School | 5:12.96 | Verified |
| 7 | DIEHL, Alexander | SO | Salem High School | 5:15.98 | Verified |
| 8 | EWERS, Brayden | JR | Vienna High School | 5:16.00 | Verified |
| 9 | GOLLAHER, Marshal | JR | Salem High School | 5:33.00 | Verified |
| 10 | DEHURT, Kurtis | JR | Salem High School | 5:36.05 | Verified |
| 11 | BROOKS, Gabriel | JR | Viburnum High School | 5:38.08 | Verified |
| 12 | WILLIAMS, Scott | FR | Rolla High School | 5:44.42 | Verified |
| 13 | PERKINS, Johnny | FR | St. James High School | 5:53.78 | Verified |
| 14 | MACE, Emerson | SO | Iberia High School | 6:04.87 | Verified |
| 15 | HUCKABEE, Micah | FR | Rolla High School | 6:05.64 | Verified |
| 16 | THIES, Nicholas | FR | Salem High School | 6:06.65 | Verified |
| 17 | BYERLEY, Ben | SO | St. Clair High School | 6:07.41 | Verified |
| 18 | DOUGLAS, Thomas | FR | Vienna High School | 6:10.00 | Unverified: |
| 19 | KROUPER-MARTINEZ, Gabriel | SR | St. Clair High School | NT | Unverified: |
| 20 | FISBECK, Logan | SR | St. Clair High School | NT | Unverified: |