| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BERRY, Kade | SR | Winfield High School | 5:30.84 | Verified |
| 2 | OSTERHOLT, Megan | JR | St. Dominic High School | 5:37.97 | Verified |
| 3 | WOYTUS, Abigail | SO | St. Dominic High School | 5:49.65 | Verified |
| 4 | LORD, Ambree | FR | Warrenton High School | 6:09.44 | Verified |
| 5 | BRANDT, Sadie | SR | St. Louis Blue Knights | 6:14.24 | Verified |
| 6 | KATHLEEN BECKEMEIER, Mary | SR | Duchesne High School | 6:14.35 | Verified |
| 7 | STEIMEL, Elle | SO | Christian High School | 6:25.39 | Verified |
| 8 | STILES, Anya | SO | St. Louis Blue Knights | 6:38.89 | Verified |
| 9 | BOSCHERT, Lily | SR | Winfield High School | 6:39.84 | Verified |