| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Louis Blue Knights | A | Gresham | Riggs | Knoerr | Plymale | 2:14.12 | Verified |
| 3 | Orchard Farm High School | A | Baer | Jewell | Pettit | Gerdes | 2:04.66 | Verified |
| 4 | St. Dominic High School | A | Pollard | Metherd | Arrowood | Burle | 1:51.09 | Verified |
| 5 | Winfield High School | A | Nelke | Stark | Reinebach | Reinebach | 1:57.91 | Verified |
| 6 | Christian High School | A | Pils | Wagner | Corwin | Painter | 2:08.67 | Verified |