| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | St. Louis Blue Knights | A | Bryant | Plymale | Nguyen | Brown | 4:37.15 | Verified |
| 4 | St. Dominic High School | A | Ryan | Zeigler | Robertson | Burle | 4:28.24 | Verified |
| 5 | Warrenton High School | A | Marschel | Morris | Rhama | Marschel | 4:30.80 | Verified |
| 6 | Orchard Farm High School | A | Bauer | Overstreet | Sharp | Franklin | NT | Unverified: |