| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Warrenton High School | A | Kackley | Guerrero | Forrest | Claiborne | 8:49.84 | Verified |
| 2 | Elsberry High School | A | Heras | Byers | Santos | Dodson | 8:52.08 | Verified |
| 3 | Orchard Farm High School | A | Ashley | Dody | Gee | Porter | 9:19.55 | Verified |
| 4 | St. Dominic High School | A | Willenbrink | Pierce | Tracy | Troesser | 9:58.92 | Verified |
| 5 | St. Louis Blue Knights | A | Ward | Meyers | Barfield | Wamsley | 10:00.01 | Verified |