| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | PETTIT, Keely | FR | Orchard Farm High School | 34.59 | Unverified: Orchard Farm JV Invitational |
| 2 | PLYMALE, Cali | FR | St. Louis Blue Knights | 31.83 | Verified |
| 3 | JEWELL, Dyamond | SR | Orchard Farm High School | 29.99 | Verified |
| 4 | HAYES, Nija | SO | Brentwood High School | 29.78 | Verified |
| 5 | PLYMALE, Cami | FR | St. Louis Blue Knights | 29.84 | Verified |
| 6 | SEBASTIAN, Mykenzy | FR | Warrenton High School | 30.03 | Verified |
| 7 | TIPPIT, Julia | SO | Elsberry High School | 32.05 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | GOFF, Breanna | SR | Duchesne High School | 28.97 | Verified |
| 2 | TALBOT, Joanna | SO | Elsberry High School | 28.67 | Verified |
| 3 | METHERD, Maya | JR | St. Dominic High School | 28.10 | Verified |
| 4 | POLLARD, Victoria | SR | St. Dominic High School | 27.60 | Verified |
| 5 | OVERKAMP, Kylie | JR | Warrenton High School | 28.05 | Verified |
| 6 | REINEBACH, Grace | SR | Winfield High School | 28.29 | Verified |
| 7 | WAGNER, Chloe | SR | Christian High School | 28.73 | Verified |
| 8 | PAINTER, Olivia | SO | Christian High School | 29.67 | Verified |