| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Montgomery County High School | A | Smith | Thomas | Thurmon | Luttrell | NT | Unverified: |
| 4 | Putnam County High School | A | Lopez | Brundage | Ream | Watt | DQ | Unverified: |
| 5 | Missouri Military Academy | B | Kahle | Burgett | Quiroga | Salinas | NT | Unverified: |
| 6 | Louisiana High School | A | Mayfield | Gamble | Black | Nichtula | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Putnam County High School | B | Lopez | Watt | Roberts | Graham | NT | Unverified: |
| 2 | Westran High School | A | Adler | Baxley | Colley | Kempker | 1:48.62 | Unverified: |
| 3 | South Shelby High School | A | Moellering | Elsen | Wiseman | Wilt | 1:42.81 | Verified |
| 4 | Mark Twain High School | A | Zumwalt | Bergthold | Preston | McLeod | 1:40.83 | Verified |
| 5 | Paris High School | A | Edwards | Goff | Shivers | Williams | 1:41.00 | Unverified: |
| 7 | Hannibal High School | A | Hall | Baker | Haynes | Jaworski | DQ | Verified |
| 8 | Salisbury High School | A | Zunguze | Noll | White | Harmon | NT | Unverified: |