| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Putnam County High School | A | Seaton | Fisher | Knowles | Dickerson | NT | Unverified: |
| 2 | Louisiana High School | A | Campbell | Ries | Chatman | McGlothin | 2:02.20 | Verified |
| 3 | South Shelby High School | A | Gaines | Roush | Nelson | Wilt | 2:01.57 | Verified |
| 4 | Palmyra High School | A | Redd | Loman | McBride | White | 1:56.00 | Unverified: |
| 5 | Hannibal High School | A | Lightle | Vandiver | Sherman | Johnson | 2:01.04 | Verified |
| 6 | Louisiana High School | B | Lentz | Sperry | Wallace | Lesley | 2:02.20 | Verified |
| 7 | Salisbury High School | A | Gladbach | Linneman | Nolke | Schiltz | 2:03.78 | Verified |
| 8 | Monroe City High School | A | Clark | Hathaway | Harsell | Lehenbauer | NT | Unverified: |