| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Putnam County High School | A | Fisher | Perkins | Calley | Knowles | NT | Unverified: |
| 3 | Salisbury High School | A | Gladbach | Linneman | Schiltz | Knight | 5:01.96 | Verified |
| 4 | Palmyra High School | A | Sheputis | Leon | Loman | White | 4:35.00 | Unverified: |
| 5 | South Shelby High School | A | Mitchell | Roush | Eleazarraraz | McWilliams | 4:44.61 | Verified |
| 6 | Mark Twain High School | A | Hudelson | Norton | Dotson | Miller | NT | Unverified: |