| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WAGNER, Sharli | SO | Marion County High School | NT | Unverified: |
| 2 | CAMPBELL, Tyonna | JR | Louisiana High School | NT | Unverified: |
| 3 | NORTON, Lucille | FR | Mark Twain High School | NT | Unverified: |
| 4 | KINNISON, Vallorie | FR | Salisbury High School | NT | Unverified: |
| 5 | BOCK, Maya | FR | Marion County High School | NT | Unverified: |
| 6 | MARTIN, Mya | FR | Monroe City High School | NT | Unverified: |
| 7 | HALEY, Lauren | FR | Mark Twain High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | CHAMORRO RUBIO, Elisabeth | JR | Putnam County High School | NT | Unverified: |
| 3 | JOHNSON, Avery | SO | Monroe City High School | NT | Unverified: |
| 4 | LIGHTLE, Kelsey | FR | Hannibal High School | 1:23.67 | Verified |
| 5 | ALLEN, Lydia | JR | Putnam County High School | 1:29.13 | Verified |
| 6 | LESLEY, Michela | FR | Louisiana High School | NT | Unverified: |
| 7 | ROBBINS, Leah | SO | Heartland Christian Academy | NT | Unverified: |
| 8 | STAPLETON, Niah | FR | Salisbury High School | NT | Unverified: |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | BREUKLANDER, Alexis | FR | Salisbury High School | 1:21.56 | Verified |
| 2 | SHERMAN, Elizabeth | FR | Hannibal High School | 1:18.60 | Verified |
| 3 | SEATON, Haven | JR | Putnam County High School | 1:15.70 | Verified |
| 5 | WILT, Madison | SO | South Shelby High School | 1:12.00 | Unverified: |
| 7 | EMMERICH, Adeline | JR | Salisbury High School | 1:18.77 | Verified |
| 8 | RILEY, Chloe | FR | Hannibal High School | 1:22.78 | Verified |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEON, Sandra | FR | Palmyra High School | 1:11.35 | Verified |
| 2 | GAINES, Hannah | FR | South Shelby High School | 1:11.00 | Unverified: |
| 3 | PEDERSEN, Jordan | SO | Louisiana High School | 1:09.73 | Verified |
| 4 | SHEPUTIS, Laurin | JR | Palmyra High School | 1:04.15 | Verified |
| 5 | HAYS, Meghan | SO | Monroe City High School | 1:08.13 | Verified |
| 6 | RODGERS, Malia | JR | Montgomery County High School | 1:10.00 | Unverified: |
| 7 | ELEAZARRARAZ, Sofia | SO | South Shelby High School | 1:11.00 | Unverified: |
| 8 | CLARK, Jaelynn | JR | Monroe City High School | 1:11.83 | Unverified: Monroe City Open #1 |