| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Putnam County High School | A | Lopez | Tomlin | Rouse | Perkins | NT | Unverified: |
| 3 | Putnam County High School | B | Lopez | Watt | Graham | Brundage | NT | Unverified: |
| 4 | Salisbury High School | A | Zunguze | Wyatt | White | Noll | NT | Unverified: |
| 5 | Palmyra High School | A | Dames | Pennewell | Mitchell | Hicks | NT | Unverified: |
| 6 | Montgomery County High School | A | Czerniewski | Luttrell | Brower | Birkner | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | South Shelby High School | A | Moellering | Wilt | Wiseman | Hammond | 47.77 | Verified |
| 4 | Hannibal High School | A | Hall | Baker | Haynes | Jaworski | 44.33 | Verified |
| 5 | Louisiana High School | A | Greene | Dzierwa | Luck | Gamble | 47.06 | Verified |
| 6 | Mark Twain High School | A | Bennett | Tobin | Zumwalt | Cruz | 49.30 | Verified |
| 7 | South Shelby High School | B | Bowen | Timbrook | Carter | Magruder | 52.77 | Unverified: |
| 8 | Missouri Military Academy | A | Burgett | Erskine | Kahle | Quiroga | 1:00.75 | Verified |