| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Marion County High School | A | Bock | Hathaway | Myers | Kindhart | NT | Unverified: |
| 4 | Putnam County High School | A | Seaton | Brundage | Knowles | Dickerson | NT | Unverified: |
| 5 | Monroe City High School | A | Foss | Hathaway | Martin | Reinberg | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Mark Twain High School | A | Boleach | Hudelson | Hawn | Shepherd | NT | Unverified: |
| 2 | Louisiana High School | A | Campbell | Ries | Chatman | McGlothin | 1:06.23 | Verified |
| 3 | Salisbury High School | A | King | Kottman | Nolke | Wales | 57.27 | Verified |
| 4 | Palmyra High School | A | Redd | Voss | McBride | White | 54.20 | Unverified: |
| 5 | South Shelby High School | A | McWilliams | Mitchell | Roush | Roush | 55.20 | Verified |
| 6 | Louisiana High School | B | Wallace | Ince | Lentz | Lesley | 1:06.23 | Verified |
| 7 | Putnam County High School | B | Wood | Miller | Webb | Fisher | NT | Unverified: |
| 8 | Monroe City High School | B | Youngblood | Johnson | Clark | Hays | NT | Unverified: |