| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | DIVELEY, Bailey | SO | Putnam County High School | 6:06.83 | Verified |
| 2 | HAYS, Ella | JR | Monroe City High School | 6:10.65 | Verified |
| 3 | CLABORN, MCKENNA | SO | Silex High School | 6:18.59 | Verified |
| 4 | JOHNSON, Avery | SO | Monroe City High School | 6:23.39 | Verified |
| 5 | GOSSEEN, Kristen | SO | Northeast (Cairo) High School | 6:33.89 | Verified |
| 6 | GITTEMEIER, Melia | FR | Northeast (Cairo) High School | 6:34.25 | Verified |
| 7 | JOHNSON, Mia | JR | Clark County High School | 6:34.65 | Verified |
| 8 | STEPHENSON, Jill | FR | Westran High School | 6:39.70 | Verified |
| 9 | SEILER, Elizabeth | JR | Macon High School | 6:40.83 | Verified |
| 10 | ARBELO, Jimena | JR | Putnam County High School | 6:53.09 | Verified |
| 11 | RUFENER, Tadara | SR | South Shelby High School | 6:57.09 | Verified |
| 12 | BUCKMAN, Shelby | SO | South Shelby High School | 7:10.68 | Verified |
| 13 | GORDAN, Avery | SO | Marceline High School | 7:39.60 | Verified |
| 14 | STONEBURNER, Lilly | SO | North Shelby High School | 7:43.64 | Verified |
| 15 | PREWITT, Aliza | JR | Westran High School | 8:52.66 | Verified |
| 16 | DARLING, Emily | FR | Marceline High School | NT | Unverified: |
| 17 | WILEY, Adrien | FR | North Shelby High School | NT | Unverified: |
| 18 | PLENGE, Irelyn | FR | Clark County High School | NT | Unverified: |