| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 4 | Atlanta High School | A | McElhaney | Nelson | Mason | Christensen | NT | Verified |
| 5 | Monroe City High School | A | Jones | Karr | Lehenbauer | Woolen | 1:56.88 | Verified |
| 6 | Marion County High School | A | Kelso | Martin | Wood | Tuley | NT | Unverified: |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clark County High School | A | Fuller | Westercamp | WHEELER | Billings | 1:42.15 | Unverified: |
| 2 | Missouri Military Academy | A | Kahle | Salinas | Brooks | Carter | 1:40.94 | Verified |
| 3 | Putnam County High School | A | Lopez | Brundage | Tomlin | Perkins | 1:37.92 | Verified |
| 4 | Milan High School | A | Ngombo | Reyes Acevedo | Linhart | Bennett | 1:36.83 | Verified |
| 5 | South Shelby High School | A | Moellering | Elsen | Wiseman | Hammond | 1:36.87 | Verified |
| 6 | Westran High School | A | Kribbs | Harlan | Stegall | Harlan | 1:39.53 | Verified |
| 7 | Macon High School | A | Carpenter | Harland | Reed | Martie | 1:41.02 | Verified |
| 8 | Marceline High School | A | Shull | Stallo | Baxter | DeWeese | 1:46.15 | Verified |