| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clark County High School | A | Lovell | IRVIN | West | Veloso | NT | Unverified: |
| 4 | Monroe City High School | A | Foss | Flick | Miller | Hays | 2:05.44 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Westran High School | A | Harvey | Wortman | Boyack | Hayes | 2:00.53 | Verified |
| 2 | South Shelby High School | A | McWilliams | Mitchell | Gaines | Roush | 1:56.19 | Verified |
| 3 | Marceline High School | A | Rodgers | Thornburg | Weese | Coe | 1:55.55 | Verified |
| 4 | Macon High School | A | Terry | McVicker | Anderson | Weimer | 1:52.75 | Verified |
| 5 | Northeast (Cairo) High School | A | Cross | Ellison | Hankins | Bailey | 1:54.00 | Verified |
| 6 | Putnam County High School | A | Seaton | Knowles | Fisher | Dickerson | 1:55.58 | Verified |
| 7 | Silex High School | A | Hall | Kuntz | Thoroughman | Weatherford | 1:57.55 | Verified |
| 8 | North Shelby High School | A | Copenhaver | Kirby | Jones | Linberger | 2:03.38 | Verified |