| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Missouri Military Academy | A | Salinas | Canonico | Miles | Powell | 5:33.93 | Verified |
| 4 | Atlanta High School | A | Nelson | Christensen | Mason | Phillips | NT | Verified |
| 5 | Milan High School | A | Wilson | Alvarez | Alcantara | Alvarez | 4:24.50 | Verified |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clark County High School | A | SHINN | Day | Westercamp | WHEELER | 3:55.74 | Verified |
| 2 | Putnam County High School | A | Roberts | Watt | Huse | Campbell-Johnson | 3:53.59 | Verified |
| 3 | Macon High School | A | Glover | Robinson | Quinn | Harland | 3:48.98 | Verified |
| 4 | South Shelby High School | A | Elsen | Wiseman | Moellering | Hammond | 3:39.49 | Verified |
| 5 | Knox County High School | A | Hayes | Jansen | Miller | Strange | 3:46.28 | Verified |
| 6 | Monroe City High School | A | Karr | Wheeler | Lehenbauer | Woolen | 3:49.81 | Verified |
| 7 | Marceline High School | A | Evans | Davis | DeWeese | Darling | 3:54.95 | Verified |