| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Silex High School | A | Auer | Isom | Klein | Pickens | 4:47.09 | Verified |
| 2 | Clark County High School | A | Plenge | Little | WEAVER | YATES | 4:44.01 | Unverified: |
| 3 | Macon High School | A | Barnett | Weimer | Weimer | McVicker | 4:36.15 | Verified |
| 4 | South Shelby High School | A | Roush | Gaines | Mitchell | McWilliams | 4:27.39 | Verified |
| 5 | Putnam County High School | A | Knowles | Knowles | Perkins | Fisher | 4:33.40 | Verified |
| 6 | North Shelby High School | A | Uhlmeyer | Copenhaver | Wiley | Parsons | NT | Verified |
| 7 | Marceline High School | A | Coe | Ewigman | Darling | Weese | 4:46.18 | Verified |