| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Shelby High School | A | Moore | Seaton | Ratliff | Vasser | 9:15.93 | Verified |
| 2 | Monroe City High School | A | Lehenbauer | Richardson | Hunter | Lorenson | 9:21.16 | Verified |
| 3 | Putnam County High School | A | Campbell-Johnson | Kaster | Ream | Watt | 9:25.01 | Verified |
| 4 | Knox County High School | A | Clark | Hayes | Miller | Campbell | 9:27.54 | Verified |
| 5 | Marion County High School | A | Britt | Schluckebier | Tuley | Wood | 9:28.57 | Verified |
| 6 | Macon High School | A | Glover | Quinn | Stephenson | Scantlin | 9:33.58 | Verified |
| 7 | Clark County High School | A | Hurt | Wardlow | McDaniel | Day | 9:38.82 | Verified |
| 8 | Marceline High School | A | Dorrell | Davis | Schmitt | Darling | 9:39.21 | Verified |
| 9 | Atlanta High School | A | Nelson | Christensen | Mason | Phillips | NT | Verified |
| 10 | Missouri Military Academy | A | Mugisha | Miles | Snyder | Quiroga | 10:56.81 | Verified |