| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WILT, Noah | SO | South Shelby High School | 2:10.20 | Verified |
| 2 | WHEELER, Jackson | SR | Monroe City High School | 2:10.26 | Verified |
| 3 | WATT, Jude | JR | Putnam County High School | 2:10.96 | Verified |
| 4 | CAMPBELL-JOHNSON, Avery | SO | Putnam County High School | 2:15.22 | Verified |
| 5 | MUGISHA, Kellan | SR | Missouri Military Academy | 2:17.57 | Verified |
| 6 | DAVIS, Cayden | SR | Marceline High School | 2:18.77 | Verified |
| 7 | DAY, Austin | SO | Clark County High School | 2:21.17 | Verified |
| 8 | MILLER, Grayson | FR | Knox County High School | 2:22.03 | Verified |
| 9 | HAYES, Collin | JR | Knox County High School | 2:22.12 | Verified |
| 10 | MILES, Ryan | SO | Missouri Military Academy | 2:22.82 | Verified |
| 11 | PHILLIPS, Roman | SR | Atlanta High School | 2:23.31 | Verified |
| 12 | WINKLER, Nolen | SO | Westran High School | 2:24.61 | Verified |
| 13 | VASSER, Nicholas | SO | South Shelby High School | 2:26.47 | Verified |
| 14 | WOOD, Mason | SO | Marion County High School | 2:28.00 | Verified |
| 15 | SCHLUCKEBIER, Rheo | SO | Marion County High School | 2:28.00 | Verified |
| 16 | HUNTER, Aidan | SO | Monroe City High School | 2:29.86 | Verified |
| 17 | WARDLOW, Isaac | SO | Clark County High School | 2:31.14 | Verified |
| 18 | DORRELL, Matthew | SR | Marceline High School | 2:33.21 | Verified |
| 19 | NELSON, Cooper | FR | Atlanta High School | 2:34.03 | Verified |
| 20 | WOHLGEMUTH, Jackson | SO | Westran High School | 2:42.01 | Verified |
| 21 | O'HARA, Devin | FR | Macon High School | 2:54.53 | Verified |
| 22 | LAMPE, Brayden | FR | Macon High School | NT | Unverified: |
| 23 | CHRISTINE, Weston | FR | North Shelby High School | NT | Unverified: |